| 1 | POWDER RIVER ENERGY CORPORATION | SUNDANCE | $176.2M | $482.7M | Unclassified |
| 2 | IVINSON MEMORIAL HOSPITAL | LARAMIE | $161.6M | $417.9M | Health Care |
| 3 | WYOMING EDUCATORS BENEFIT TRUST | CHEYENNE | $139.2M | $49.5M | Unclassified |
| 4 | UNIVERSITY OF WYOMING FOUNDATION | LARAMIE | $113.6M | $980.2M | Education |
| 5 | HIGH WEST ENERGY INC | PINE BLUFFS | $87.0M | $168.2M | Unclassified |
| 6 | HIGH PLAINS POWER INC | RIVERTON | $85.1M | $156.5M | Unclassified |
| 7 | POWELL VALLEY HEALTH CARE INC | POWELL | $82.7M | $34.4M | Health Care |
| 8 | LOWER VALLEY ENERGY INC | AFTON | $80.2M | $318.7M | Unclassified |
| 9 | COMMUNITY FOUNDATION OF JACKSON HOLE | JACKSON | $75.2M | $170.9M | Philanthropy, Voluntarism & Grantmaking Foundations |
| 10 | HOLDFAST TR | JACKSON | $63.3M | $1.4B | Environment |
| 11 | VOLUNTEERS OF AMERICA INC | SHERIDAN | $56.7M | $68.8M | Unclassified |
| 12 | DELTA DENTAL PLAN OF WYOMING | CHEYENNE | $51.8M | $26.3M | Unclassified |
| 13 | JACKSON HOLE CLASSICAL SCHOOL INC | JACKSON | $45.9M | $52.6M | Education |
| 14 | NATIONAL OUTDOOR LEADERSHIP SCHOOL | LANDER | $43.0M | $116.0M | Environment |
| 15 | HOT SPRINGS COUNTY HOSPITAL DIST | THERMOPOLIS | $40.4M | $46.1M | Health Care |
| 16 | GRAND TETON NATIONAL PARK FOUNDATION | MOOSE | $34.0M | $52.0M | Philanthropy, Voluntarism & Grantmaking Foundations |
| 17 | BUFFALO BILL MEMORIAL ASSOCIATION | CODY | $32.5M | $155.6M | Arts, Culture & Humanities |
| 18 | CHEYENNE FRONTIER DAYS INC | CHEYENNE | $26.8M | $46.8M | Unclassified |
| 19 | WYRULEC COMPANY | LINGLE | $26.2M | $57.0M | Unclassified |
| 20 | WYOMING COMMUNITY FOUNDATION | LARAMIE | $23.0M | $230.8M | Philanthropy, Voluntarism & Grantmaking Foundations |
| 21 | WYO COMPLEX | CASPER | $22.5M | $43.9M | Recreation & Sports |
| 22 | WHEATLAND RURAL ELECTRIC ASSOCIATION | WHEATLAND | $20.4M | $43.2M | Unclassified |
| 23 | COMMUNITY HEALTH CENTER OF CENTRAL WYOMING INC | CASPER | $20.2M | $19.1M | Health Care |
| 24 | TETON SCIENCE SCHOOLS | JACKSON | $19.6M | $53.9M | Environment |
| 25 | CHEYENNE LEADS | CHEYENNE | $18.3M | $44.1M | Unclassified |
| 26 | CARBON POWER & LIGHT INC | SARATOGA | $17.6M | $60.1M | Unclassified |
| 27 | BIG HORN RURAL ELECTRIC COMPANY | BASIN | $17.4M | $44.9M | Unclassified |
| 28 | NIOBRARA ELECTRIC ASSOCIATION INC | LUSK | $17.1M | $40.4M | Public & Societal Benefit |
| 29 | WYOMING MACHINERY COMPANY EMPLOYEE MEDICAL BENEFIT TRUST | CASPER | $15.9M | $2.5M | Unclassified |
| 30 | GRAND TETON MUSIC FESTIVAL INC | JACKSON | $15.4M | $33.3M | Arts, Culture & Humanities |
| 31 | WYOMING CHILD & FAMILY DEVELOPEMENT INC | GUERNSEY | $14.8M | $10.9M | Education |
| 32 | DECENTRALIZED WIRELESS FOUNDATION INC | JACKSON | $14.4M | $19.4M | Community Improvement & Capacity Building |
| 33 | ARK REGIONAL SERVICES INC | LARAMIE | $14.4M | $9.8M | Housing & Shelter |
| 34 | BRIDGER VALLEY ELECTRIC ASSOCIATION INC | MOUNTAIN VIEW | $14.3M | $30.0M | Public & Societal Benefit |
| 35 | WYOMING CATHOLIC COLLEGE | LANDER | $14.1M | $15.5M | Education |
| 36 | CENTRAL WYOMING COUNSELING CENTER | CASPER | $13.9M | $18.5M | Mental Health & Crisis Intervention |
| 37 | RIVERPEAK HEALTH | RIVERTON | $12.7M | $21.1M | Health Care |
| 38 | ST JOHNS HEALTH FOUNDATION | JACKSON | $12.1M | $24.7M | Health Care |
| 39 | HIGH COUNTRY BEHAVIORAL HEALTH | AFTON | $11.3M | $13.9M | Mental Health & Crisis Intervention |
| 40 | WESTERN STATES LEARNING CORPORATION | CHEYENNE | $11.1M | $10.1M | Education |
| 41 | ST JOSEPHS CHILDRENS HOME | TORRINGTON | $10.8M | $23.5M | Mental Health & Crisis Intervention |
| 42 | COMMUNITY CENTER FOR THE ARTS | JACKSON | $10.6M | $36.4M | Arts, Culture & Humanities |
| 43 | COMMUNITY ENTRY SERVICES INC | RIVERTON | $10.0M | $9.8M | Employment |
| 44 | CATHEDRAL HOME FOR CHILDREN | LARAMIE | $9.9M | $17.4M | Human Services |
| 45 | JACKSON HOLE LAND TRUST | JACKSON | $9.8M | $42.5M | Environment |
| 46 | CLOUD PEAK COUNSELING CENTER | CODY | $9.6M | $13.0M | Mental Health & Crisis Intervention |
| 47 | CENTRAL WYOMING COLLEGE FOUNDATION | RIVERTON | $9.2M | $32.9M | Unclassified |
| 48 | CENTRAL WYOMING HOSPICE PROGRAM | CASPER | $8.9M | $14.3M | Unclassified |
| 49 | WESTERN FUTURES FUND INC | SHERIDAN | $8.7M | $3.9M | Philanthropy, Voluntarism & Grantmaking Foundations |
| 50 | LARAMIE COUNTY LIBRARY FOUNDATION INC | CHEYENNE | $8.1M | $12.2M | Education |